Phosphoserine(Biotinylated)
pSER-Biotinylated
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Antigen Preparation
A chemically linked phosphoserine.
Background
Protein phosphorylation is involved in cell signaling pathways. These cascades are mediated by two types of kinases: serine/threonine kinases which phosphorylate serine and threonine amino acid side chains and tyrosine kinases which phosphorylate tyrosine amino acid side chains. This process is regulated by kinases and phosphatases.
PURIFICATION
The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
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SPECIFICITY
This antibody recognizes phospho-serine (phospho-Ser) only. It does not cross react with phospho-Tyrosine or phospho-Threonine
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FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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