Antigen Preparation
A synthetic peptide corresponding to C-term of ubiquitin protein.
Background
"Ubiquitin (Ub) is a highly conserved protein of approximately 8.5 kDa. It plays a very important role in regulated non-lysosomal ATP dependent protein degradation. The protein to be degraded is covalently attached to C-term of ubiquitin, and the ubiquinated protein is then selectively degraded by a 26S complex, multicatalytic cytosolic and nuclear protease termed proteasome. The Ub-proteasome proteolytic pathway, which is a complex process, is implicated to be of great importance for regulating numerous cellular processes. "
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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