Antigen Preparation
A synthetic peptide corrsponding to the internal segment of human Osteopontin
Background
"Osteopontin (OPN or Early T-Lymphocyte Activation-1: Eta-1), also referred to as transformation-associated secreted phosphoprotein, bone sialoprotein I, and minopotin, is a highly phosphorylated and glycosylated phosphoprtein that is expressed in many tissues. It is acidic, calcium-binding, cell surface-binding sequences (RGD)-containing protein was originally isolated from bone matrix. Osteopontin has been found in kidney, placenta, blood vessels. It is over-expressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma."
Applications/Suggested Working Dilutions
|
|
|
Immunoprecipitation
2-5 µg/ml
|
|
Flow cytometry
Not tested
|