SUMO-3/Sentrin-3
Small ubiquitin-related modifier 3; Sentrin-3
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Antigen Preparation
"A synthetic peptide derived from C-terminus of human Sentrin-3 protein. This sequence is identical to human, mouse and rat."
Background
"Sentrin-3 also known as SUMO-3 is a 103-amino acids peptide that has high homologous to Sentrin-1/SUMO-1. This novel ubiquitin-like protein can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. "
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
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SPECIFICITY
"This antibody reacts with SUMO-3 protein. It cross-reacts to human, mouse and rat. The other species are not tested."
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FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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