Antigen Preparation
A synthetic peptide derived from internal domain of human NFκB p65
Background
"NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) is a protein complex that controls the transcription of DNA. NF-κB is important in regulating cellular responses because it belongs to the category of ""rapid-acting"" primary transcription factors. NF kappa B is a heterodimer consisting of a 50kDa DNA binding subunit and a 65kDa transactivating subunit. In unstimulated cells, the NF-κB dimers are sequestered in the cytoplasm by a family of inhibitors, called IκBs (Inhibitor of κB). Activation of the NF-κB is initiated by the signal-induced degradation of IκB proteins. Subsequent to cell stimulation, IκB undergoes phosphorylation, ubiquitination and degradation by a proteosome-dependent pathway, allowing nuclear translocation of the active dimeric NFκB transcription factor. NF-κB complex enters the nucleus where it can 'turn on' the expression of specific genes that have DNA-binding sites for NF-κB nearby. The activation of these genes by NF-κB then leads to the given physiological response, for example, an inflammatory or immune response, a cell survival response, or cellular proliferation."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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