Neurofilaments (200kDa&68kDa)
200kDa-Neurofilament, heavy polypeptide; NFH, 68kDa-Neurofilament, light chain; NFL
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Antigen Preparation
human glial fibrillary acidic protein
Background
"Neurofilaments, NF heavy chain (NFH, 200 kda) and light chain (NFL, 68 kDa), are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. Detection of NFH or NGL, and the subunits of NF, may serve as a biomarker for axonal degeneration. The degree of axonal degeneration is related to the amount of NF detected in the cerebrospinal fluid (CSF) and the blood. Neurofilament light (NF-L) levels are elevated in Alzheimer’s Disease."
PURIFICATION
The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
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SPECIFICITY
This antbody recognizes human Neurofilaments (200kDa&68kDa). The other species not tested.
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FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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