Antigen Preparation
Synthetic peptide corresponding to human PKN
Background
"PKN1, protein kinase N1, belongs to the protein kinase C superfamily. It is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. It also can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate PKN1, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. "
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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