Antigen Preparation
A synthetic peptide corresponding to residues surrounding Ser 282 of human ELK-1.
Background
"Elk1 belongs to ets proto-oncogene family. It functions as a transcription activator. Elk-1, a major nuclear target of extracellular signal-regulated kinases, is a strong transactivator of serum-responsive element (SRE) driven gene expression. Elk1 forms SRF-dependent ternary complexes with the SRE. It is important because a key event in the response of cells to proliferative signals is the rapid, transient induction of the c-fos proto-oncogene, which is mediated through the SRE in the fos promoter"
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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