Antigen Preparation
"A synthetic peptide surrounding to the epitope -RGYVP-- with phosphorylation sites at Tyr641 of STAT6 protein from human, mouse and rat origins."
Background
"STAT proteins (Signal transducer and activator of transcription) belong to a family of cytoplasmic transcription factors that can be phosphorylated by a ligand binding to its cell surface receptor. Activation by phosphorylation of STAT6 at tyrosine 641 upon the IL-4 (possibly IL-13) ligand binding to its receptor induces dimerization, nuclear translocation and DNA binding. STAT6 is widely expressed in human tissues. It exhibits elevated expression in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung, and placenta. STAT6 mediates the IL-4 signal and is essential for the proper development of adaptive immunity"
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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