STAT3 (pY705)
Signal transducer and activator of transcription 3 (STAT3)
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Antigen Preparation
"A synthetic peptide surrounding to the epitope -APYLK-- with phosphorylation sites at Tyr705 of STAT3 protein from human, mouse, rat, chicken dog and bovine origins."
Background
"STAT proteins (Signal transducer and activator of transcription) belong to a family of cytoplasmic transcription factors that can be phosphorylated by a lignad binding to its cell surface receptor. The phosphorylation of STAT3 induces STAT dimerization, nuclear translocation and DNA binding. Stat3 is constitutively activated in a number of human tumors. Stat3-activated transcription seems to be regulated by serine phosphorylation at Ser727 and tyrosine phosphorylation at Tyr705."
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
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SPECIFICITY
This antibody recognizes STAT3 (pY705) with a phosphorylated sites of Tyr705. It does not cross-react with non-phosphospecific peptide.
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FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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