SOX-9 (pS181)
SRY (sex determining region Y)-box 9
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Antigen Preparation
"A synthetic peptide surrounding the epitope –RKSVK- with a single phosphorylation site Ser181. This sequence is identical among human, rat, mouse, bovine, chicken, and dog."
Background
"The SOX9 is a member of the SOX [Sry-related high-mobility group (HMG) box] family of HMG DNA-binding domain transcription factors and is required for the development and differentiation of multiple cell lineages. SOX9 is expressed in primary prostate cancers in vivo, at a higher frequency in recurrent prostate cancer and in prostate cancer cell lines (LNCaP, CWR22, PC3, and DU145). It has been reported that the cAMP/PKA stimulation induces SOX-9 nuclear translocation via facilitating its nuclear import by the phosphorylation of SOX-9 at Ser64 and Ser181. SOX9 in prostate basal cells supports the development and maintenance of the luminal epithelium and that a subset of prostate cancer cells may escape basal cell requirements through SOX9 expression."
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
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SPECIFICITY
This antibody recognizes ~56 kDa of human SOX-9 protein at the phosphorylation site of Ser181. It does not cross react to non-phospho SOX-9. It also reacts with mouse and rat. The other species are not tested.
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FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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