Neurofibromin(pS2741)
NF1 neurofibromin 1
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Antigen Preparation
"A synthetic peptide surrounding the epitope –PTSPY- with a phosphorylation site of Ser2741 of human neurofibromin. This sequence is identical among human, rat, mouse, chicken, bovine and dog"
Background
"The neurofibromatosis type 1 (NF1) tumor suppressor (neurofibromin) is thought to play crucial roles in cellular Ras- and cAMP-dependent kinase (PKA)-associated signals. The absence of or alteration of the neurofibromin protein may lead to neurofibromatosis disease. Recently, a cellular neurofibromin-associating protein, NG,NG-dimethylarginine dimethylaminohydrolase (DDAH)has been identified as a cellular NO/NOS regulator which increases PKA phosphorylation of native neurofibromin in a dose-dependent manner. The PKA accessibility of neurofibromin regulated via DDAH interaction may modulate the cellular function of neurofibromin that is implicated in NF1-related pathogenesis."
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
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SPECIFICITY
"This antibody recognizes ~240 kDa of human neurofibromin protein at the phosphorylation site of Ser 2741. It does not cross react to non-phospho NF1.This antibody also reacts with mouse, rat, chicken, bovine and dog. The other species are not tested."
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FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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