Antigen Preparation
"A synthetic peptide surrounding to the epitope -EYYK- with dual phosphorylation sites at Tyr1007 and Tyr1008 of JAK2 from human, mouse and rat origins ."
Background
"The Janus tyrosine kinase family is consists of four members, JAK1, JAK2, JAK3 and Tyk2. Upon the cytokine ligand binding to its the receptors, the dimerization of the receptors occurs followed by phosphorylation of the receptors. The phosphorylation of receptors recruites the tyrosine kinase physically associated to the dimerized receptors, and phosphorylate the kinase, thus activates the signal transducer and activator of thranscription (STAT1, 3, 5) and the other signal proteins."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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