JunD (pS255)
Jun D proto-oncogene; AP-1
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Antigen Preparation
A synthetic peptide of human JunD with a phosphrylation site Serine 255.
Background
"JunD is a member of the jun proto-oncogene family. This protein has been proposed to protect cells from p53-dependent senescence and apoptosis. Alternative translation initiation site usage results in the production of different isoforms. Truncated c-Jun and JunD proteins containing the C-terminus recognize the same DNA sequences which are defined as the PEA1/AP1 binding sequence or TPA response element (TRE). Both can trans-activate a promoter including the TRE, and this activation is further enhanced by c-fos. "
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
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SPECIFICITY
This antibody recognizes JunD with the phosphorylation site Serine 255. It does not cross-react with non-phosphospecific peptide.
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FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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