CD8
Cluster of Differentiation 8
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Antigen Preparation
A recombinant protein derived from extracellular domain (22-182aa) was used as an immunogen.
Background
"The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. Multiple transcript variants encoding different isoforms have been found for this gene, such as CD8a, CD8b.This protein is present on virtually all peripheral blood T cells and thymocytes. The CD8 plays an important role in coupling antigen recognition to several intracellular signal transduction pathways."
PURIFICATION
The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
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SPECIFICITY
This antibody recognizes human CD8 protein. The other species are not tested.
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FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
0.5-5 µg/106 cells
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