Antigen Preparation
"A panel of synthetic peptides corresponding to the predicted multiple phosphorylation sites, including S121, Y147, S156. Those sequences are identical to human, rat and mouse."
Background
"The PLAC1, a placenta-specific gene, which encodes a putative cell surface protein, is highly expressed in placenta, testis, cancer cell lines and lung tumors. PLAC1 is exclusively expressed by cells of trophoblastic lineage in the mouse, and maps to a region of the X-chromosome known to be important in placenta growth. PLAC1 is also expressed in human hepatocellular cancer tissues as well as in several other types of cancer tissues and/or tumor cell lines. PLAC1 represents a new class of tumor associated antigen with restricted expression in placenta and cancer tissues, which may serve as a target for cancer vaccination. The phosphorylation of PLAC1 by kinases CDK5, PKC may be involved in tumor associated process. "
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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