p56-LCK (LSK) (pY505)
Lymphocyte-specific protein tyrosine kinase; LSK; p56lck
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Antigen Preparation
"A synthetic peptide derived from Carboxyl-terminus of p56lck protein with a phosphorylation site at Tyr 505 from human, rat, bovine and dog origin."
Background
"Lymphocyte specific protein tyrosine kinase (LCK) belongs to Src family protein-tyrosine kinase. It is essential for T-cell development and function. The activity p56lck is regulated by phosphorylation and dephosphorylation of two critical tyrosine residues Tyr394 and Tyr505. Tyr394 and Tyr505 are autophosphorylated after p56lck activation, whereas phosphorylation of Tyr505 is believed to be due to p50csk which negatively modulates P56lck activity. Autophosphorylation at Tyr505 of P56lck may represent an accessory mechanism for the down-regulation of the tyrosine kinase activity of P56lck."
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
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SPECIFICITY
This antibody recognizes p56lck with a phosphorylated site of Tyrosine 505. It does not cross-react with non-phosphospecific peptide.
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FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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