IkBa (pS32/36) (NFKB1A)
NFKBI, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
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Antigen Preparation
"A synthetic peptide corresponding to the epitope –DSGLDSM- at dual phosphorylation sites serine 32 and serine 36 of human IkBalpha protein. This sequence is identical within human, mouse, rat, dog and bovine."
Background
"There are six members in the IkB family, in which IkBa is first protein induced by NF-kB activation. NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. External stimuli such as TNF or other cytokines initiates a signal transduction cascade that leads to the activation of IkB-kinase complex that specifically phosphorylates IkBa on Serine-32 and Serine-36. Phosphorylation of these sites leads to ubiquitination of IkBa and subsequent degradation by the 26 S proteasome. "
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
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SPECIFICITY
This antibody recognizes ~42 kDa of human IkBa at dual phosphorylation sites of Ser32/Ser36. It does not recognize non-phosphorylated peptides. This antibody also reacts with rat and mouse. The other species are not tested.
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FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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