VASA (pS43)
VASA Protein, DEAD-box protein 4(DDX4), Mab4611
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Antigen Preparation
"A synthetic peptide corresponding to the epitope –GGSGG- with the phosphorylated serine of human VASA protein. This sequence is identical within human, rat, mouse."
Background
"The VASA gene, originally identified in Drosophila, encodes a member of the DEAD box family of ATP-dependent RNA helicases. The expression of VASA in invertebrate and vertebrate species is restricted to germ cells and is required for gemr cell formation. VASA is therefore a highly specific marker of germ cells. This protein is located in cytoplasmic and is present only in migratory primordial germ cells in the region of the gonadal ridge. On testicular sections, VASA expression is the highest in spermatogonia, reduced in spermatocytes, low in spermatids and absent in sperm. In the ovary, VASA expression is the highest in oogonia but persists throughout oogenesis. VASA has a glycine-rich N-terminus with multiple repeats of an RGG motif believed to function in RNA binding. The phosphorylation of VASA at N-terminus may regulate the translation of intricate mRNAs that are essential for differentiation."
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
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SPECIFICITY
"This antibody recognizes ~76 kDa of VASA protein at a phosphorylation site serine 43. It does not recognize non-phosphorylated peptides. This antibody also reacts with human, mouse and rat. The other species are not tested."
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FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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