Antigen Preparation
"A synthetic peptide surrounding to the epitope -DTSDP-- with phosphorylation sites at Ser385 of PTEN protein from human, mouse and rat origins."
Background
"The phosphatase and tensin homologue (PTEN) tumor suppressor is a phosphatidylinositol D3-phosphatase that is frequently mutated in tumor cells. PTEN is itself regulated by phosphorylation on multiple serine and threonine residues in its C terminus. Casein kinase 2 (CK2) and Glycogen synthase kinase 3beta (GSK3beta), ATK/PI3K are involved in PTEN phosphorylation at sites of S370, S380, T382, T383, S385. The PTEN phosphorylation may play critical roles in the tumor suppressor functions. "
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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