Antigen Preparation
"A synthetic peptide corresponding to N-term of human beta-Actin protein. This sequence is identical in human, mouse, rat, bovine and chicken."
Background
"The two major cytoskeletal proteins implicated in cell motility are actin and myosin. There are three α-actin (skeletal, cardiac, and smooth muscle), one β-actin (Î²ï€ non-muscle), and two γ-actins (γ-smooth muscle and γ-non-muscle). Antibody to β-actin provides a specific and useful tool in studying the intracellular distribution of β-actin and the static and dynamic aspects of the cytoskeleton. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This beta-actin is a major constituent of the contractile apparatus and one of the two non-muscle cytoskeletal actins."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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