Antigen Preparation
A synthetic peptide corrsponding to the internal segment of human CD105
Background
"CD105 is also known as Endoglin.This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds to the beta1 and beta3 peptides with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. This gene may also be involved in preeclampsia and several types of cancer. Human engoglin is a type I integral membrane protein with large extracellular domain, a single hydrophobic transmembrane domain, and short cytosolic domain. Cd105 is expressed at a low levels in resting endothelial cells, but highly expressed in vascular endothelial cells at sites of angiogenesis such as tumor vessels, healing wounds, during embryogenesis. "
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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