Antigen Preparation
A synthetic peptide derived from N-terminus of human alpha skeletal muscle isoforms of actin.
Background
"The Actin protein, a component of the eukaryotic cytoskeleton, is highly conserved between multiple species. This ubiquitous protein involved in the formation of filaments that are a major component of the cytoskeleton. Interaction with myosin provides the basis of muscular contraction and many aspects of cell motility. The levels of the Actin protein may vary between different cell types, but are consistent within a given cell type, and for this reason, Actin protein levels are considered an internal control for protein loading."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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