Antigen Preparation
A synthetic peptide corrsponding to the internal segment of human ERK1
Background
"The extracellular signal-regulated kinases ½ (ERK1/2) are also known as MAPK (Mitogen-activated protein kinases) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (ERK1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines and is an important target in the diagnosis and treatment of cancer. The phosphorylation of MAPK at Thr202 and Tyr 204 are correlated to the progression and metastatic potential of tumor cells."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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