Antigen Preparation
A synthetic peptide corrsponding to the N-terminus of human Amyloid beta
Background
"Amyloid beta peptides are derived from the amyloid precursor protein (APP) which is cleaved by beta and gamma secretases. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. The aggregation of the amyloid beta peptide into oligomers or fibrils is now implicated as a key process associated with progression of AD, the most common cause of dementia in senior population. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy)."
Applications/Suggested Working Dilutions
|
|
|
Immunoprecipitation
2-5 µg/ml
|
|
Flow cytometry
Not tested
|