Ki-67
MKI67; antigen KI-67; proliferation-related Ki-67 antigen
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Antigen Preparation
A synthetic peptide derived from human Ki-67 protein
Background
"Ki-67, a proliferation marker is a nuclear protein that is associated with and may be necessary for cellular proliferation. It can be used as a biomarker with Bcl-2 (an apoptosis inhibitor), P53 and Pax 2 for immunohistostaining in carcinomas diagnosis. The differences in the immunocytochemical expression of those markers are correlated to the results with tumor grade and stage for a further accurate diagnosis. "
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
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SPECIFICITY
"This antbody recognizes human, mice and rat Ki-67 protein. The other species not tested."
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FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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